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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ETV5 Polyclonal antibody (13011-1-AP)

    WB analysis using 13011-1-AP (same clone as 13011-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13011-1-AP (ETV5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ETV5 Polyclonal antibody (13011-1-AP)

    WB analysis using 13011-1-AP (same clone as 13011-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13011-1-AP (ETV5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ETV5 Polyclonal antibody (13011-1-AP)

    IP experiment of HeLa using 13011-1-AP (same clone as 13011-1-PBS)

    IP result of anti-ETV5 (IP:13011-1-AP, 4ug; Detection:13011-1-AP 1:6000) with HeLa cells lysate 960 ug. This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ETV5 Polyclonal antibody (13011-1-AP)

    IHC staining of mouse brain using 13011-1-AP (same clone as 13011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13011-1-AP (ETV5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ETV5 Polyclonal antibody (13011-1-AP)

    IHC staining of mouse brain using 13011-1-AP (same clone as 13011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13011-1-AP (ETV5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ETV5 Polyclonal antibody (13011-1-AP)

    IF Staining of PC-3 using 13011-1-AP (same clone as 13011-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 13011-1-AP (ETV5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ETV5 Polyclonal antibody, PBS Only

ETV5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13011-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ERM, Ets related protein ERM, ETS translocation variant 5, Ets-related protein ERM

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13011-1-PBS targets ETV5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3708

Product name: Recombinant human ETV5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 160-510 aa of BC007333

Sequence: SGHAPAAGPVQGVGPAPAPHSLPEPGPQQQTFAVPRPPHQPLQMPKMMPENQYPSEQRFQRQLSEPCHPFPPQPGVPGDNRPSYHRQMSEPIVPAAPPPPQGFKQEYHDPLYEHGVPGMPGPPAHGFQSPMGIKQEPRDYCVDSEVPNCQSSYMRGGYFSSSHEGFSYEKDPRLYFDDTCVVPERLEGKVKQEPTMYREGPPYQRRGSLQLWQFLVTLLDDPANAHFIAWTGRGMEFKLIEPEEVARRWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCDPDALFSMAFPDNQRPFLKAESECHLSEEDTLPLTHFEDSPAYLLDMDRCSSLPYAEGFAY

相同性解析による交差性が予測される生物種
Full Name ets variant 5
Calculated molecular weight 510 aa, 58 kDa
Observed molecular weight70 kDa
GenBank accession numberBC007333
Gene Symbol ETV5
Gene ID (NCBI) 2119
RRIDAB_2278092
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP41161
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney

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