Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 21 publications below |
| IHC | See 8 publications below |
| IF | See 4 publications below |
| ChIP | See 1 publications below |
Product Information
10684-1-AP targets ETV4 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0984 Product name: Recombinant human ETV4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-207 aa of BC007242 Sequence: MYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPFGPKGGYSY 相同性解析による交差性が予測される生物種 |
| Full Name | ets variant 4 |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 61-65 kDa |
| GenBank accession number | BC007242 |
| Gene Symbol | ETV4 |
| Gene ID (NCBI) | 2118 |
| RRID | AB_2100984 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43268 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ETV4 antibody 10684-1-AP | Download protocol |
| WB protocol for ETV4 antibody 10684-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription. | ||
Dev Cell ATXN1 protein family and CIC regulate extracellular matrix remodeling and lung alveolarization.
| ||
Nat Commun Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5. | ||
Proc Natl Acad Sci U S A Erk1/2 inactivation promotes a rapid redistribution of COP1 and degradation of COP1 substrates. | ||
Oncogene HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer
|