Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11721-1-AP targets ETV3 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2301 Product name: Recombinant human ETV3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-143 aa of BC022868 Sequence: MKAGCSIVEKPEGGGGYQFPDWAYKTESSPGSRQIQLWHFILELLQKEEFRHVIAWQQGEYGEFVIKDPDEVARLWGRRKCKPQMNYDKLSRALRYYYNKRILHKTKGKRFTYKFNFNKLVMPNYPFINIRSSGKIQTLLVGN 相同性解析による交差性が予測される生物種 |
| Full Name | ets variant 3 |
| Calculated molecular weight | 512 aa, 57 kDa |
| Observed molecular weight | 57 kDa |
| GenBank accession number | BC022868 |
| Gene Symbol | ETV3 |
| Gene ID (NCBI) | 2117 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41162 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ETV3 is a transcriptional repressor that belongs to the ETS family of transcription factors, which bind a common DNA sequence core motif (GGAA) via their ETS domains. ETV3 is preferentially expressed in mature DC including tissue-derived migratory DC (migDC) and facilitates their homeostatic maturation and CCR7-dependent migration. Phosphorylation of ETV3 regulated transcription by preventing its binding to DNA at promoters for several thousand genes, including some involved in negative feedback regulation of itself and of upstream signals (PMID: 36543959, 41678619). Western and IP analysis detected the specific band with an apparent MW of 57 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ETV3 antibody 11721-1-AP | Download protocol |
| WB protocol for ETV3 antibody 11721-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |