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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ETS2 Polyclonal antibody

ETS2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12280-1-AP

Synonyms

ETS2, ETS2IT1, Protein C ets 2

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 12280-1-AP

    WB analysis of K-562 using 12280-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 12280-1-AP

    WB analysis of PC-3 using 12280-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12280-1-AP

    WB analysis of HeLa using 12280-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 12280-1-AP

    WB analysis of MCF-7 using 12280-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 12280-1-AP

    WB analysis of mouse kidney using 12280-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12280-1-AP

    IP experiment of HeLa using 12280-1-AP

    IP result of anti-ETS2 (IP:12280-1-AP, 3ug; Detection:12280-1-AP 1:600) with HeLa cells lysate 5000ug.

  • IHC staining of mouse spleen using 12280-1-AP

    IHC staining of mouse spleen using 12280-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ETS2 antibody included
  • IHC staining of mouse spleen using 12280-1-AP

    IHC staining of mouse spleen using 12280-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ETS2 antibody included
  • IHC staining of rat liver using 12280-1-AP

    IHC staining of rat liver using 12280-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ETS2 antibody included
  • IHC staining of rat liver using 12280-1-AP

    IHC staining of rat liver using 12280-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ETS2 antibody included

Tested Applications

Positive WB detected inK-562 cells, HeLa cells, MCF-7 cells, mouse kidney tissue, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse spleen tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ETS2 Ready-To-Use IHC Kit. ETS2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

12280-1-AP targets ETS2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ETS2 fusion protein Ag2929 相同性解析による交差性が予測される生物種
Full Name v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)
Calculated molecular weight 469 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC017040
Gene symbol ETS2
Gene ID (NCBI) 2114
RRIDAB_10804755
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ETS2 antibody 12280-1-APDownload protocol
IHC protocol for ETS2 antibody 12280-1-APDownload protocol
IP protocol for ETS2 antibody 12280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

The overexpression of Tipe2 in CRC cells suppresses survival while endogenous Tipe2 accelerates AOM/DSS induced-tumor initiation.

Authors - Yan Li
View Article
humanWB

Cell Death Dis

A distal super-enhancer activates oncogenic ETS2 via recruiting MECOM in inflammatory bowel disease and colorectal cancer

Authors - Yongheng Chen
View Article