Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of MCF-7 using 21045-1-AP
WB result of ESRP1 antibody (21045-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ESRP1 transfected MCF-7 cells.
WB analysis of COLO 320 using 21045-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 21045-1-AP (ESRP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 21045-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 21045-1-AP (ESRP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 21045-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 21045-1-AP (ESRP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 21045-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 21045-1-AP (ESRP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of COLO 320 using 21045-1-AP
IP result of anti-ESRP1 (IP:21045-1-AP, 4ug; Detection:21045-1-AP 1:300) with COLO 320 cells lysate 4000ug.
IHC staining of mouse colon using 21045-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 21045-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 21045-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 21045-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 21045-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 21045-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ESRP1 antibody (21045-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

ESRP1 Polyclonal antibody

ESRP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21045-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

RBM35A, RNA binding motif protein 35A, RNA binding protein 35A, RNA-binding motif protein 35A, RNA-binding protein 35A

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COLO 320 cells, mouse lung tissue, MCF-7 cells
Positive IP detected in	COLO 320 cells
Positive IHC detected in	human lung tissue, mouse colon tissue, human bowen disease **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 9 publications below
IHC	See 1 publications below
IF	See 3 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

21045-1-AP targets ESRP1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15386 Product name: Recombinant human ESRP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-226 aa of BC099916 Sequence: EKELILLFWKVVDLANKKVGQLHEVLVRPDQLELTEDCKEETKIDVESLSSASQLDQALRQFNQSVSNELNIGVGTSFCLCTDGQLHVRQILHPEASKKNVLLPECFYSFFDLRKEFKKCCPGSPDIDKLDVATMTEYLNFEKSSSVSRYGASQVEDMGNIILAMISEPYNHRFSDPERVNYKFESGTCSKMELIDDNTV 相同性解析による交差性が予測される生物種
Full Name	epithelial splicing regulatory protein 1
Calculated molecular weight	681 aa, 76 kDa
Observed molecular weight	76 kDa
GenBank accession number	BC099916
Gene Symbol	ESRP1
Gene ID (NCBI)	54845
RRID	AB_2878801
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6NXG1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ESRP1, also named as Epithelial splicing regulatory protein 1, is a 681 amino acid protein, which belongs to the ESRP family. ESRP1 as a mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. ESRP1 specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2 and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). ESRP1 exists various isoforms and molecular weight of each isoform is 76 kDa, 67 kDa, and 73 kDa.

Protocols

Product Specific Protocols
IF protocol for ESRP1 antibody 21045-1-AP	Download protocol
IHC protocol for ESRP1 antibody 21045-1-AP	Download protocol
IP protocol for ESRP1 antibody 21045-1-AP	Download protocol
WB protocol for ESRP1 antibody 21045-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Cancer Res MEN1 Promotes Ferroptosis by Disrupting CD44 Alternative Splicing to Suppress Lung Cancer Authors - Ting Pan View Article
human	WB	Nucleic Acids Res ESRP1 controls biogenesis and function of a large abundant multiexon circRNA Authors - Dawei Liu KD Validated View Article
human	WB	J Exp Clin Cancer Res ESRP1 exerts anti-tumor role by promoting ferroptosis in diffuse-type gastric cancer Authors - Kang Xu View Article
human	WB	Cell Death Differ Splicing factor derived circular RNA circUHRF1 accelerates oral squamous cell carcinoma tumorigenesis via feedback loop. Authors - Wei Zhao View Article
	RIP	Mol Ther circCAMSAP1 Promotes Tumor Growth in Colorectal Cancer via the miR-328-5p/E2F1 Axis. Authors - Chi Zhou KD Validated View Article
human	WB	Cell Death Discov Circ-NOLC1 promotes epithelial ovarian cancer tumorigenesis and progression by binding ESRP1 and modulating CDK1 and RhoA expression. Authors - Shuo Chen KD Validated View Article

Validation Data Gallery

WB analysis of MCF-7 using 21045-1-AP

WB analysis of COLO 320 using 21045-1-AP

WB analysis of mouse lung using 21045-1-AP

WB analysis of COLO 320 using 21045-1-AP

WB analysis of COLO 320 using 21045-1-AP

IP experiment of COLO 320 using 21045-1-AP

IHC staining of mouse colon using 21045-1-AP

IHC staining of human lung using 21045-1-AP

IHC staining of human lung using 21045-1-AP

IHC staining of mouse colon using 21045-1-AP

IHC staining of human bowen disease using 21045-1-AP

IF Staining of HeLa using 21045-1-AP

ESRP1 Polyclonal antibody

ESRP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MEN1 Promotes Ferroptosis by Disrupting CD44 Alternative Splicing to Suppress Lung Cancer

ESRP1 controls biogenesis and function of a large abundant multiexon circRNA

ESRP1 exerts anti-tumor role by promoting ferroptosis in diffuse-type gastric cancer

Splicing factor derived circular RNA circUHRF1 accelerates oral squamous cell carcinoma tumorigenesis via feedback loop.

circCAMSAP1 Promotes Tumor Growth in Colorectal Cancer via the miR-328-5p/E2F1 Axis.

Circ-NOLC1 promotes epithelial ovarian cancer tumorigenesis and progression by binding ESRP1 and modulating CDK1 and RhoA expression.

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