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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ESPN Polyclonal antibody (20717-1-AP)

    WB analysis using 20717-1-AP (same clone as 20717-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20717-1-AP (ESPN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20717-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using ESPN Polyclonal antibody (20717-1-AP)

    WB analysis of Neuro-2a using 20717-1-AP (same clone as 20717-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20717-1-AP (ESPN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20717-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ESPN Polyclonal antibody (20717-1-AP)

    IHC staining of human ovary cancer using 20717-1-AP (same clone as 20717-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20717-1-AP (ESPN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20717-1-PBS in a different storage buffer formulation.

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ESPN Polyclonal antibody, PBS Only

ESPN Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20717-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

LP2654, Espin, DFNB36

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20717-1-PBS targets ESPN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name espin
Calculated molecular weight 92 kDa
Observed molecular weight 30-33 kDa, 110 kDa
GenBank accession numberNM_031475
Gene Symbol ESPN
Gene ID (NCBI) 83715
RRIDAB_10858327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDB1AK53
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ESPN, also named as DFNB36, is a multifunctional actin-bundling protein. ESPN plays a major role in regulating the organization, dimensions, dynamics and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in variouS mechanosensory and chemosensory cells. Defects in ESPN are the cause of deafness autosomal recessive type 36 (DFNB36). Defects in ESPN are the cause of deafness autosomal dominant without vestibular involvement (DFNAWVI). The antibody is specific to ESPN.