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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ESPL1 Polyclonal antibody (22261-1-AP)

    WB analysis using 22261-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22261-1-AP (ESPL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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ESPL1 Polyclonal antibody

ESPL1 Polyclonal Antibody for WB, ELISA
Cat No. 22261-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Caspase like protein ESPL1, ESP1, KIAA0165, SEPARASE, SEPARIN

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, Jurkat cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22261-1-AP targets ESPL1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name extra spindle pole bodies homolog 1 (S. cerevisiae)
Calculated molecular weight 233 kDa
Observed molecular weight170 kDa
GenBank accession numberNM_012291
Gene Symbol ESPL1
Gene ID (NCBI) 9700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14674
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ESPL1 (Extra spindle poles-like 1 protein), also known as Separin, Separase, a cysteine endopeptidase, plays a vital role in the stable binding between sister chromatids before anaphase and their timely separation during anaphase, which is the key to chromosome inheritance (PMID: 34512713). ESPL1 expression is elevated and predicts poor outcomes in patients with various types of cancer, including breast cancer, glioma and endometrial cancer (PMID: 36465268). Full-length ESPL1 decreased after 30 min resulting in a ~ 170 kDa N-terminal (ESPL1N−term) and a ~ 60 kDa C-terminal polypeptide (ESPL1CC−term), reflecting ESPL1 activation and auto-cleavage (PMID: 30429481)

Protocols

Product Specific Protocols
WB protocol for ESPL1 antibody 22261-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols