Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells, PC-3 cells, DU 145 cells, A2780 cells, SKOV-3 cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86027-1-RR targets ESM1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ESM1 fusion protein Ag3019 相同性解析による交差性が予測される生物種 |
| Full Name | endothelial cell-specific molecule 1 |
| Calculated molecular weight | 184 aa, 20 kDa |
| Observed molecular weight | 14 kDa |
| GenBank accession number | BC011989 |
| Gene Symbol | ESM1 |
| Gene ID (NCBI) | 11082 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NQ30 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ESM1, also known as endocan or endothelial cell-specific molecule 1, is a protein-coding gene that encodes a secreted proteoglycan primarily expressed in endothelial cells of human tissues, including lung, kidney, and vascular systems. The protein consists of a 20 kDa core protein with a dermatan sulfate chain, forming a 50 kDa mature proteoglycan. ESM1 plays a crucial role in endothelial cell proliferation, migration, and angiogenesis (blood vessel formation). It modulates vascular development and maintains endothelial homeostasis. Overexpression of ESM1 is observed in cancers such as gastric, cervical, and lung tumors, where it promotes angiogenesis and tumor progression via pathways like VEGF/ERK signaling. Elevated serum ESM1 levels are associated with poor clinical outcomes in cancer patients.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ESM1 antibody 86027-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |