ESM1 Polyclonal antibody
ESM1 Polyclonal Antibody for IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IP, ELISA
Conjugate
Unconjugated
Cat no : 12355-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IP detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Sample-dependent, check data in validation data gallery | |
Product Information
12355-1-AP targets ESM1 in IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ESM1 fusion protein Ag3019 相同性解析による交差性が予測される生物種 |
| Full Name | endothelial cell-specific molecule 1 |
| Calculated molecular weight | 184 aa, 20 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC011989 |
| Gene symbol | ESM1 |
| Gene ID (NCBI) | 11082 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ESM1 is a secreted protein mainly expressed in the endothelial cells. The deduced 184-amino acid protein is cysteine rich with an N-terminal hydrophobic signal sequence. Expression of ESM1 was tested by Northern blot analysis and RT-PCR and was found to be constitutive in a HUVEC cell line, but not in any other cell line tested. Constitutive ESM1 expression in human tissue was restricted to lung.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ESM1 antibody 12355-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
