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Species Reactivity

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  • Featured Product
  • KD/KO Validated

ESD Polyclonal antibody

ESD Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15244-1-AP

Synonyms

ESD, Esterase D, FGH, S formylglutathione hydrolase

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 15244-1-AP

    WB analysis of K-562 using 15244-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15244-1-AP

    WB analysis of HeLa using 15244-1-AP

    WB result of ESD antibody (15244-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ESD transfected HeLa cells.

  • WB analysis of HeLa using 15244-1-AP

    WB analysis of HeLa using 15244-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 15244-1-AP

    WB analysis of K-562 using 15244-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inK-562 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Sample-dependent, check data in validation data gallery

Product Information

15244-1-AP targets ESD in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ESD fusion protein Ag7457 相同性解析による交差性が予測される生物種
Full Name esterase D/formylglutathione hydrolase
Calculated molecular weight 31 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC001169
Gene symbol ESD
Gene ID (NCBI) 2098
RRIDAB_2101686
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ESD antibody 15244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols