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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERLEC1 Polyclonal antibody (29773-1-AP)

    WB analysis using 29773-1-AP (same clone as 29773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29773-1-AP (ERLEC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29773-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ERLEC1 Polyclonal antibody (29773-1-AP)

    IHC staining of mouse brain using 29773-1-AP (same clone as 29773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29773-1-AP (ERLEC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29773-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ERLEC1 Polyclonal antibody (29773-1-AP)

    IHC staining of mouse brain using 29773-1-AP (same clone as 29773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29773-1-AP (ERLEC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29773-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29773-1-PBS targets ERLEC1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERLEC1 fusion protein Ag31135 相同性解析による交差性が予測される生物種
Full Name chromosome 2 open reading frame 30
Calculated molecular weight 55 kDa
Observed molecular weight50-55 kDa
GenBank accession numberNM_015701
Gene Symbol ERLEC1
Gene ID (NCBI) 27248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DZ1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Endoplasmic Reticulum Lectin 1 (ERLEC1)is also named as C2orf30, XTP3TPB. It may function in endoplasmic reticulum quality control and endoplasmic reticulum-associated degradation (ERAD) of both non-glycosylated proteins and glycoproteins. ERLEC1 has 3 isoforms with MW of 54 kDa, and can be detected as 50-55 kDa.