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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERK5 Polyclonal antibody (11164-1-AP)

    WB analysis using 11164-1-AP (same clone as 11164-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11164-1-AP (MAPK7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11164-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using ERK5 Polyclonal antibody (11164-1-AP)

    IHC staining of human stomach using 11164-1-AP (same clone as 11164-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11164-1-AP (ERK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11164-1-PBS in a different storage buffer formulation.

ERK5 Polyclonal antibody, PBS Only

ERK5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11164-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ERK 5, MAPK7, BMK-1, EC:2.7.11.24, ERK-5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11164-1-PBS targets ERK5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1648

Product name: Recombinant human MAPK7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC009963

Sequence: MAEPLKEEDGEDGSAEPPGPVKAEPAHTAASVAAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVSSARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDILRPTVPYGEFKSVYVVLDLMESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTSPAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGTPSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAALRHPFLAKY

相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 7
Calculated molecular weight 89 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC009963
Gene Symbol MAPK7
Gene ID (NCBI) 5598
RRIDAB_2877749
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13164
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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