Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of NIH/3T3 using 16443-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 16443-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 16443-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 16443-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16443-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16443-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 16443-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 16443-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16443-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 16443-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16443-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human kidney using 16443-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 16443-1-AP (ERK1/2 Antibody) at dilution of 1:50.
IHC staining of human kidney using 16443-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 16443-1-AP (ERK1/2 Antibody) at dilution of 1:50.
IF Staining of HeLa using 16443-1-AP
Immunofluorescent analysis of HeLa cells using 16443-1-AP (ERK1/2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16443-1-AP

Publications(462)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ERK2, MAPK1, EC:2.7.11.24, ERK, ERK 2

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (462)

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	NIH/3T3 cells, A431 cells, mouse kidney tissue, HEK-293 cells, HeLa cells, PC-3 cells, rat kidney tissue, mouse brain tissue, rat brain tissue
Positive IHC detected in	human liver tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 439 publications below
IHC	See 7 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

16443-1-AP targets ERK1/2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, sheep, eriocheir sinensis
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9772 Product name: Recombinant human ERK1/2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-360 aa of BC017832 Sequence: YTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase 1
Calculated molecular weight	360 aa, 41 kDa
Observed molecular weight	38-44 kDa
GenBank accession number	BC017832
Gene Symbol	ERK2
Gene ID (NCBI)	5594
RRID	AB_10603369
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P28482
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Publications

Species	Application	Title
human	WB	J Hematol Oncol Exosomes released by hepatocarcinoma cells endow adipocytes with tumor-promoting properties. Authors - Shihua Wang View Article
mouse	WB	Gastroenterology Macrophage-derived cathepsin S remodels the extracellular matrix to promote liver fibrogenesis Authors - Tao Zuo View Article
mouse,human	WB	Cancer Commun (Lond) PTPRO represses colorectal cancer tumorigenesis and progression by reprogramming fatty acid metabolism. Authors - Weixing Dai View Article
human	WB	Nat Chem Biol Nucleophilic covalent ligand discovery for the cysteine redoxome Authors - Ling Fu View Article
human	WB	Theranostics Obatoclax and LY3009120 Efficiently Overcome Vemurafenib Resistance in Differentiated Thyroid Cancer. Authors - Wei-Jun Wei View Article
human	WB	J Exp Clin Cancer Res Long non-coding RNA UBE2CP3 enhances HCC cell secretion of VEGFA and promotes angiogenesis by activating ERK1/2/HIF-1α/VEGFA signalling in hepatocellular carcinoma. Authors - Jinduan Lin View Article

Validation Data Gallery

WB analysis of NIH/3T3 using 16443-1-AP

WB analysis of rat kidney using 16443-1-AP

WB analysis of A431 using 16443-1-AP

WB analysis of mouse kidney using 16443-1-AP

WB analysis of HEK-293 using 16443-1-AP

WB analysis of HeLa using 16443-1-AP

WB analysis of PC-3 using 16443-1-AP

IHC staining of human liver using 16443-1-AP

IHC staining of human liver using 16443-1-AP

IHC staining of human kidney using 16443-1-AP

IHC staining of human kidney using 16443-1-AP

IF Staining of HeLa using 16443-1-AP

ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Publications

Exosomes released by hepatocarcinoma cells endow adipocytes with tumor-promoting properties.

Macrophage-derived cathepsin S remodels the extracellular matrix to promote liver fibrogenesis

PTPRO represses colorectal cancer tumorigenesis and progression by reprogramming fatty acid metabolism.

Nucleophilic covalent ligand discovery for the cysteine redoxome

Obatoclax and LY3009120 Efficiently Overcome Vemurafenib Resistance in Differentiated Thyroid Cancer.

Long non-coding RNA UBE2CP3 enhances HCC cell secretion of VEGFA and promotes angiogenesis by activating ERK1/2/HIF-1α/VEGFA signalling in hepatocellular carcinoma.

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