Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 11257-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1/2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11257-1-AP
WB result of ERK1/2 antibody (11257-1-AP, 1:1000) with HeLa and HEK293 cells.
WB analysis using 11257-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1/2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11257-1-AP
WB result of ERK1/2 antibody (11257-1-AP, 1:2000) with si-Control and si-ERK1/2 transfected HeLa cells.
IP experiment of HEK-293 using 11257-1-AP
IP result of anti-ERK1/2 (IP:11257-1-AP, 4ug; Detection:11257-1-AP 1:3000) with HEK-293 cells lysate 1440 ug.
IHC staining of human colon cancer using 11257-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11257-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11257-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 11257-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of mouse brain using 11257-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ERK1/2 antibody (11257-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 11257-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ERK1/2 antibody (11257-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HeLa using 11257-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ERK1/2 (11257-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 11257-1-AP

Publications(842)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Synonyms

ERK, ERK1, ERK2, EC:2.7.11.24, ERK-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 555 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (842)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	PC-12 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, C6 cells, mouse brain tissue, mouse lung tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue
Positive IF/ICC detected in	HEK-293 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 743 publications below
IHC	See 18 publications below
IF	See 27 publications below
IP	See 2 publications below
ELISA	See 1 publications below
CoIP	See 4 publications below

Product Information

11257-1-AP targets ERK1/2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine, chicken, zebrafish, sheep, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1759 Product name: Recombinant human ERK1/2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-379 aa of BC013992 Sequence: MAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPGVLEAP 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase 3
Calculated molecular weight	43 kDa
Observed molecular weight	38-44 kDa
GenBank accession number	BC013992
Gene Symbol	ERK1
Gene ID (NCBI)	5595
RRID	AB_2139822
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P27361
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Protocols

Product Specific Protocols
FC protocol for ERK1/2 antibody 11257-1-AP	Download protocol
IF protocol for ERK1/2 antibody 11257-1-AP	Download protocol
IHC protocol for ERK1/2 antibody 11257-1-AP	Download protocol
IP protocol for ERK1/2 antibody 11257-1-AP	Download protocol
WB protocol for ERK1/2 antibody 11257-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Signal Transduct Target Ther A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics Authors - Zhiheng Rao View Article
Human	WB	Signal Transduct Target Ther Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells Authors - Pengju Wang View Article
human	WB	Mol Cancer CMTM6 overexpression confers trastuzumab resistance in HER2-positive breast cancer Authors - Fei Xing View Article
human	WB	Mol Cancer A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer Authors - Runjie Song View Article
human	WB	Gastroenterology M6A-mediated upregulation of FZD10 regulates liver cancer stem cells properties and lenvatinib resistance through WNT/β-catenin and Hippo signaling pathways Authors - Jinghan Wang View Article
mouse	WB,IP,IHC,IF,FC (Intra),ELISA,CoIP	Adv Mater Ultrasound-Activated Sonophage Synergizes Sonodynamic Therapy and Saltoptosis for Solid Tumor Eradication. Authors - Kerong Chen View Article

Validation Data Gallery

WB analysis using 11257-1-AP

WB analysis of HEK-293 using 11257-1-AP

WB analysis using 11257-1-AP

WB analysis of HeLa using 11257-1-AP

IP experiment of HEK-293 using 11257-1-AP

IHC staining of human colon cancer using 11257-1-AP

IHC staining of human colon cancer using 11257-1-AP

IHC staining of human breast cancer using 11257-1-AP

IHC staining of human breast cancer using 11257-1-AP

IF Staining of mouse brain using 11257-1-AP

IF Staining of HEK-293 using 11257-1-AP

FC experiment of HeLa using 11257-1-AP

ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics

Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells

CMTM6 overexpression confers trastuzumab resistance in HER2-positive breast cancer

A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer

M6A-mediated upregulation of FZD10 regulates liver cancer stem cells properties and lenvatinib resistance through WNT/β-catenin and Hippo signaling pathways

Ultrasound-Activated Sonophage Synergizes Sonodynamic Therapy and Saltoptosis for Solid Tumor Eradication.

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