Validation Data Gallery
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Tested Applications
| Positive WB detected in | PC-12 cells, HeLa cells, Jurkat cells, RAW 264.7 cells, ROS1728 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 77 publications below |
| IHC | See 1 publications below |
Product Information
67170-1-Ig targets ERK1/2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse, rat, rabbit, chicken |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | mitogen-activated protein kinase 3 |
| Calculated molecular weight | 38-43 kDa |
| GenBank accession number | NM_002746 |
| Gene Symbol | ERK1 |
| Gene ID (NCBI) | 5595 |
| RRID | AB_2882466 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P27361 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
Publications
| Species | Application | Title |
|---|---|---|
J Hepatol Nucleolin lactylation contributes to intrahepatic cholangiocarcinoma pathogenesis via RNA splicing regulation of MADD | ||
Nat Cell Biol Defective prelamin A processing promotes unconventional necroptosis driven by nuclear RIPK1 | ||
Nat Commun SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation | ||
Nat Commun A glucose kinase-independent HK2 activity prevents TNF-induced cell death by phosphorylating RIPK1. | ||