Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, HUVEC cells, COLO 320 cells, HepG2 cells, MOLT-4 cells, HSC-T6 cells, RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60929-1-Ig targets ERK1/2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | mitogen-activated protein kinase 1 |
| Calculated molecular weight | 360 aa, 41 kDa |
| Observed molecular weight | 38-44 kDa |
| GenBank accession number | BC017832 |
| Gene Symbol | ERK2 |
| Gene ID (NCBI) | 5594 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P28482 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ERK1 and ERK2 belong to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalyzed the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with a molecular mass of 38-44 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERK1/2 antibody 60929-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |