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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERGIC2 Polyclonal antibody (11927-1-AP)

    WB analysis using 11927-1-AP (same clone as 11927-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11927-1-AP (ERGIC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ERGIC2 Polyclonal antibody (11927-1-AP)

    WB analysis of HeLa using 11927-1-AP (same clone as 11927-1-PBS)

    WB result of ERGIC2 antibody (11927-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC2 transfected HeLa cells. This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ERGIC2 Polyclonal antibody (11927-1-AP)

    IP experiment of HepG2 using 11927-1-AP (same clone as 11927-1-PBS)

    IP result of anti-ERGIC2 (IP:11927-1-AP, 4ug; Detection:11927-1-AP 1:1000) with HepG2 cells lysate 2900ug. This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ERGIC2 Polyclonal antibody (11927-1-AP)

    IHC staining of human prostate cancer using 11927-1-AP (same clone as 11927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11927-1-AP (ERGIC2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ERGIC2 Polyclonal antibody (11927-1-AP)

    IF Staining of HepG2 using 11927-1-AP (same clone as 11927-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11927-1-AP (ERGIC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ERGIC2 Polyclonal antibody, PBS Only

ERGIC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11927-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11927-1-PBS targets ERGIC2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2603

Product name: Recombinant human ERGIC2 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-377 aa of BC000887

Sequence: MRRLNRKKTLSLVKELDAFPKVPESYVETSASGGTVSLIAFTTMALLTIMEFSVYQDTWMKYEYEVDKDFSSKLRINIDITVAMKCQYVGADVLDLAETMVASADGLVYEPTVFDLSPQQKEWQRMLQLIQSRLQEEHSLQDVIFKSAFKSTSTALPPREDDSSQSPNACRIHGHLYVNKVAGNFHITVGKAIPHPRGHAHLAALVNHESYNFSHRIDHLSFGELVPAIINPLDGTEKIAIDHNQMFQYFITVVPTKLHTYKISADTHQFSVTERERIINHAAGSHGVSGIFMKYDLSSLMVTVTEEHMPFWQFFVRLCGIVGGIFSTTGMLHGIGKFIVEIICCRFRLGSYKPVNSVPFEDGHTDNHLPLLENNTH

相同性解析による交差性が予測される生物種
Full Name ERGIC and golgi 2
Calculated molecular weight 377 aa, 43 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC000887
Gene Symbol ERGIC2
Gene ID (NCBI) 51290
RRIDAB_2098440
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RQ1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ERGIC2, also named as Endoplasmic reticulum-Golgi intermediate compartment protein 2 or PTX1, is a 377 amino acid protein, which belongs to the ERGIC family. ERGIC2 is ubiquitously expressed in many tissues. ERGIC2 localizes in the endoplasmic reticulum-Golgi intermediate compartment membrane and may play a role in transport between endoplasmic reticulum and Golgi. ERGIC2 is involved in protein transportation, and participated in the elongation factor 1 alpha regulated mechanisms.

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