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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ERCC6L2 Polyclonal antibody (27028-1-AP)

    WB analysis of PC-3 using 27028-1-AP (same clone as 27028-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 27028-1-AP (ERCC6L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27028-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ERCC6L2 Polyclonal antibody (27028-1-AP)

    WB analysis of HepG2 using 27028-1-AP (same clone as 27028-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27028-1-AP (ERCC6L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27028-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ERCC6L2 Polyclonal antibody (27028-1-AP)

    IHC staining of human stomach cancer using 27028-1-AP (same clone as 27028-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27028-1-AP (ERCC6L2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27028-1-PBS in a different storage buffer formulation.

ERCC6L2 Polyclonal antibody, PBS Only

ERCC6L2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27028-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

DNA excision repair protein ERCC-6-like 2, DNA repair and recombination protein RAD26-like, EC:3.6.4.-, RAD26L, SR278

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27028-1-PBS targets ERCC6L2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19348

Product name: Recombinant human C9orf102 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 358-712 aa of BC140702

Sequence: KRELATGRKAMQRLAKKMSGWFLRRTKTLIKDQLPKKEDRMVYCSLTDFQKAVYQTVLETEDVTLILQSSEPCTCRSGQKRRNCCYKTNSHGETVKTLYLSYLTVLQKVANHVALLQAASTSKQQETLIKRICDQVFSRFPDFVQKSKDAAFETLSDPKYSGKMKVLQQLLNHCRKNRDKVLLFSFSTKLLDVLQQYCMASGLDYRRLDGSTKSEERLKIVKEFNSTQDVNICLVSTMAGGLGLNFVGANVVVLFDPTWNPANDLQAIDRAYRIGQCRDVKVLRLISLGTVEEIMYLRQIYKQQLHCVVVGSENAKRYFEAVQGSKEHQGELFGIHNLFKFRSQGSCLTKDILEV

相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 102
Calculated molecular weight 712 aa, 81 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC140702
Gene Symbol ERCC6L2
Gene ID (NCBI) 375748
RRIDAB_2880729
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ5T890
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ERCC6L2 , also known as RAD26L or C9orf102 belongs to the SNF2/RAD54 helicase family and is expressed in bone marrow. C9orf102 may be involved in early DNA damage response. C9orf102 is phosphorylated by NEK6. C9orf102 exists some isoforms with the molecular weight 177, 80, 60, 40 kDa.

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