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ERCC6L Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.313-20 ng/mL

Cat no : MP02582-2

Print datasheet

Synonyms

ATP-dependent helicase ERCC6-like, DNA excision repair protein ERCC-6-like, EC:3.6.4.12, PICH, PLK1-interacting checkpoint helicase

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02582-2, Human ERCC6L Recombinant Matched Antibody Pair - PBS only. 86688-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8223. 86688-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

    Sandwich ELISA standard curve of MP02582-2

    Sandwich ELISA standard curve of MP02582-2, Human ERCC6L Recombinant Matched Antibody Pair - PBS only. 86688-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8223. 86688-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • The mean ERCC6L concentration was determined to be 355.50 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 346.30 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 214.50 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 409.90 ng/mL in K-562 cell extract based on a 1.2 mg/mL extract load and 318.20 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

    Sandwich ELISA sample test of MP02582-2

    The mean ERCC6L concentration was determined to be 355.50 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 346.30 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 214.50 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 409.90 ng/mL in K-562 cell extract based on a 1.2 mg/mL extract load and 318.20 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02582-2 targets ERCC6L in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.313-20 ng/mL (Sandwich ELISA);

Capture antibody: ERCC6L Recombinant monoclonal antibody, PBS Only (Capture) 86688-1-PBS (251669A8). 100 μg. Concentration 1 mg/ml.

Detection antibody: ERCC6L Recombinant monoclonal antibody, PBS Only (Detector) 86688-2-PBS (251669C3). 100 μg. Concentration 1 mg/ml.

Alternative ERCC6L matched antibody pairs: MP02582-1

Matched pair protein standard: Ag8223

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 86688-1-PBS
Tested Reactivity human
Immunogen ERCC6L fusion protein Ag8223
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 6-like
Synonyms ATP-dependent helicase ERCC6-like, DNA excision repair protein ERCC-6-like, EC:3.6.4.12, PICH, PLK1-interacting checkpoint helicase
GenBank accession numberBC008808
Gene symbol ERCC6L
UniprotQ2NKX8
Gene ID (NCBI) 54821
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 86688-2-PBS
Tested Reactivity human
Immunogen ERCC6L fusion protein Ag8223
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 6-like
Synonyms ATP-dependent helicase ERCC6-like, DNA excision repair protein ERCC-6-like, EC:3.6.4.12, PICH, PLK1-interacting checkpoint helicase
GenBank accession numberBC008808
Gene symbol ERCC6L
UniprotQ2NKX8
Gene ID (NCBI) 54821
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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