Validation Data Gallery
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Tested Applications
| Positive WB detected in | DU 145 cells, HeLa cells, PC-3 cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
24291-1-AP targets ERCC6/CSB in WB, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18009 Product name: Recombinant human ERCC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1144-1493 aa of BC127104 Sequence: DESIDEKLGLSYKRERPSQAQTEAFWENKQMENNFYKHKSKTKHHSVAEEETLEKHLRPKQKPKNSKHCRDAKFEGTRIPHLVKKRRYQKQDSENKSEAKEQSNDDYVLEKLFKKSVGVHSVMKHDAIMDGASPDYVLVEAEANRVAQDAPKALRLSRQRCLGAVSGVPTWTGHRGISGAPAGKKSRFGKKRNSNFSVQHPSSTSPTEKCQDGIMKKEGKDNVPEHFSGRAEDADSSSGPLASSSLLAKMRARNHLILPERLESESGHLQEASALLPTTEHDDLLVEMRNFIAFQAHTDGQASTREILQEFESKLSASQSCVFRELLRNLCTFHRTSGGEGIWKLKPEYC 相同性解析による交差性が予測される生物種 |
| Full Name | excision repair cross-complementing rodent repair deficiency, complementation group 6 |
| Calculated molecular weight | 1493 aa, 168 kDa |
| Observed molecular weight | 170 kDa |
| GenBank accession number | BC127104 |
| Gene Symbol | ERCC6/CSB |
| Gene ID (NCBI) | 2074 |
| RRID | AB_2879476 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q03468 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ERCC6/CSB antibody 24291-1-AP | Download protocol |
| WB protocol for ERCC6/CSB antibody 24291-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Conserved intronic secondary structures with concealed branch sites regulate alternative splicing of poison exons | ||
Int J Mol Sci A Gene Expression Signature to Predict Nucleotide Excision Repair Defects and Novel Therapeutic Approaches. | ||
Front Mol Biosci Blockage of ERCC6 Alleviates Spinal Cord Injury Through Weakening Apoptosis, Inflammation, Senescence, and Oxidative Stress.
| ||
Front Oncol Studies on the functionality of the TC-NER ERCC6-M1097V protein variant frequently found in Louisiana patients with PCa upon UV damage.
| ||
Life Sci Alliance Elevated glucose increases genomic instability by inhibiting nucleotide excision repair. |