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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERCC5 Monoclonal antibody (67055-1-Ig)

    WB analysis using 67055-1-Ig (same clone as 67055-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67055-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using ERCC5 Monoclonal antibody (67055-1-Ig)

    WB analysis of Daudi using 67055-1-Ig (same clone as 67055-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67055-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using ERCC5 Monoclonal antibody (67055-1-Ig)

    WB analysis of Ramos using 67055-1-Ig (same clone as 67055-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67055-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using ERCC5 Monoclonal antibody (67055-1-Ig)

    WB analysis of COLO 320 using 67055-1-Ig (same clone as 67055-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67055-1-PBS in a different storage buffer formulation.

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ERCC5 Monoclonal antibody, PBS Only

ERCC5 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67055-1-PBS
Clone No.1G2C9

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

COFS3, ERCC5, ERCM2, UVDR, XPG, XPGC

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67055-1-PBS targets ERCC5 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERCC5 fusion protein Ag28561 相同性解析による交差性が予測される生物種
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 5
Calculated molecular weight 1186 aa, 133 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC031522
Gene Symbol ERCC5
Gene ID (NCBI) 2073
RRIDAB_2882366
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP28715
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The human genes correcting DNA repair defects are termed excision-repair cross-complementing or ERCC genes. The ERCC5 gene corrects the excision repair deficiency of Chinese hamster ovary cell line UV135 of complementation group 5. The human ERCC5 gene product is a structure-specific endonuclease required for making the 3-prime incision during DNA nucleotide excision-repair (NER). It also plays an important role in regulating DNA excision repair, removal of bulky lesions caused by environmental chemicals or UV light [PMID:22815677]. The calculated molecular weight of ERCC5 is 133 kDa, but the modified ERCC5 protein is about 200 kDa.