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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of COLO 320 using 10580-1-AP (same clone as 10580-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of HEK-293 using 10580-1-AP (same clone as 10580-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of HeLa using 10580-1-AP (same clone as 10580-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of HeLa using 10580-1-AP (same clone as 10580-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of A431 using 10580-1-AP (same clone as 10580-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of mouse colon using 10580-1-AP (same clone as 10580-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of HT-1080 using 10580-1-AP (same clone as 10580-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of HeLa using 10580-1-AP (same clone as 10580-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ERCC3 Polyclonal antibody (10580-1-AP)

    WB analysis of HEK-293 using 10580-1-AP (same clone as 10580-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A431 cells using ERCC3 Polyclonal antibody (10580-1-AP)

    IP experiment of A431 using 10580-1-AP (same clone as 10580-1-PBS)

    IP result of anti-ERCC3 (IP:10580-1-AP, 4ug; Detection:10580-1-AP 1:500) with A431 cells lysate 1200ug. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using ERCC3 Polyclonal antibody (10580-1-AP)

    IHC staining of human stomach using 10580-1-AP (same clone as 10580-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10580-1-AP (ERCC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ERCC3 Polyclonal antibody, PBS Only

ERCC3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10580-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

BTF2, BTF2 p89, DNA 3'-5' helicase/translocase XPB, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10580-1-PBS targets ERCC3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0876

Product name: Recombinant human ERCC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 483-782 aa of BC008820

Sequence: LIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEDLAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSSRSKAPSKHVHPLFKRFRK

相同性解析による交差性が予測される生物種
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing)
Calculated molecular weight 89 kDa
Observed molecular weight 89 kDa
GenBank accession numberBC008820
Gene Symbol ERCC3
Gene ID (NCBI) 2071
RRIDAB_2262174
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP19447
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Excision repair cross-complementing 3/xeroderma pigmentosum B (ERCC3/XPB) DNA helicase, is an essential ATP-dependent DNA helicase that functions as an integral component of the transcription factor IIH complex. ERCC3 is involved in initiation of basal transcription and global genome repair as well as in transcription-coupled repair (TCR) [PMID:19942596]. At the cellular level, a defect in ERCC3 results in high UV sensitivity, reduced post-UV RNA synthesis recovery and decreased DNA repair due to defective NER [PMID:16947863].

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