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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ERCC2 Polyclonal antibody (10818-1-AP)

    WB analysis of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)

    WB result of ERCC2 antibody (10818-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERCC2 transfected HEK-293 cells. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ERCC2 Polyclonal antibody (10818-1-AP)

    WB analysis of HeLa using 10818-1-AP (same clone as 10818-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ERCC2 Polyclonal antibody (10818-1-AP)

    WB analysis of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using ERCC2 Polyclonal antibody (10818-1-AP)

    WB analysis of K-562 using 10818-1-AP (same clone as 10818-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ERCC2 Polyclonal antibody (10818-1-AP)

    IP experiment of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)

    IP result of anti-ERCC2 (IP:10818-1-AP, 4ug; Detection:10818-1-AP 1:1000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ERCC2 Polyclonal antibody (10818-1-AP)

    IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ERCC2 Polyclonal antibody (10818-1-AP)

    IHC staining of human cervical cancer using 10818-1-AP (same clone as 10818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ERCC2 Polyclonal antibody (10818-1-AP)

    IHC staining of human cervical cancer using 10818-1-AP (same clone as 10818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ERCC2 Polyclonal antibody (10818-1-AP)

    IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 10x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ERCC2 Polyclonal antibody (10818-1-AP)

    IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 40x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

ERCC2 Polyclonal antibody, PBS Only

ERCC2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10818-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

BTF2 p80, COFS2, CXPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10818-1-PBS targets ERCC2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1038

Product name: Recombinant human ERCC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC008346

Sequence: MRELKRTLDAKGHGVLEMPSGTGKTVSLLALIMAYQRAYPLEVTKLIYCSRTVPEIEKVIEELRKLLNFYEKQEGEKLPFLGLALSSRKNLCIHPEVTPLRFGKDVDGKCHSLTASYVRAQYQHDTSLPHCRFYEEFDAHGREVPLPAGIYNLDDLKALGRRQGWCPYFLARYSILHANVVVYSYHYLLDPKIADLVSKELARKAVVVFDEAHNIDNVCIDSMSVNLTRRTLDRCQGNLETLQKTVLRIKETDEQRLRDEYRRLVEGLREASAARETDAHLANPVLPDEVLQEAVPGSIRT

相同性解析による交差性が予測される生物種
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 2
Calculated molecular weight 87 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC008346
Gene Symbol ERCC2
Gene ID (NCBI) 2068
RRIDAB_2231330
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP18074
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Excision-repair complementing defective in Chinese hamster 2(ERCC2), belongs to the nucleotide excision repair pathway, is a component of the TFIIH transcription complex and required for the association of the CDK-activating kinase(CAK) subcomplex with TFIIH. Since TFIIH is involved in both DNA repair and cell cycle progression, ERCC2 is implicated to play an integral role in the nucleotide excision repair pathway. Also it involves in the regulation of vitamin-D receptor activity. As a part of the mitotic spindle-associated MMXD complex, ERCC2 has a role in chromosome segregation.

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