Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293T cells, Jurkat cells, U2OS cells, MCF-7 cells, MDA-MB-231 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87639-1-RR targets ERBB2IP in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg5491 Product name: Recombinant human ERBIN protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 950-1100 aa of NM_001253697.1 Sequence: HNPEEPNIIRGPTSGPQSAPQIYGPPQYNIQYSSSAAVKDTLWHSKQNPQIDHASFPPQLLPRSESTENQSYAKHSANMNFSNHNNVRANTAYHLHQRLGPARHGEMWAISPNDRLIPAVTRSTIQRQSSVSSTASVNLGDPGSTRRAQIP 相同性解析による交差性が予測される生物種 |
| Full Name | erbb2 interacting protein |
| Calculated molecular weight | 158 kDa |
| Observed molecular weight | 150-180 kDa |
| GenBank accession number | NM_001253697.1 |
| Gene Symbol | ERBB2IP |
| Gene ID (NCBI) | 55914 |
| RRID | AB_3745668 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96RT1-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ERBB2IP is a cytoplasmic protein that contains leucine-rich repeats (LRR) and PSD95/Dlg1/zo-1 (PDZ) domain (thus named a LAP protein). ERBB2IP interacts, via its single PDZ domain, specifically with ErbB2, but not with ErbB3, ErbB4, or EGFR. It is colocalized with ErbB2 at the basolateral membranes of epithelial cells. In addition, ERBB2IP also interacts with PSD‐95, Lano and with three p120‐related catenins, eBPAG1 and the β4 subunit of the α6β4 integrin, two major components of hemidesmosomes. The molecular weight of ERBB2IP was predicted 150~180 kDa by Western Blot.(PMID: 25288731,PMID: 24252174,PMID: 19289088)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERBB2IP antibody 87639-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |