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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HER2/ErbB2,p185-Specific Recombinant antibody (84046-1-RR)

    WB analysis using 84046-1-RR (same clone as 84046-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84046-1-RR (ERBB2,p185-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using HER2/ErbB2,p185-Specific Recombinant antibody (84046-1-RR)

    WB analysis of MCF-7 using 84046-1-RR (same clone as 84046-1-PBS)

    WB result of HER2/ErbB2,p185-Specific antibody (84046-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HER2/ErbB2,p185-Specific transfected MCF-7 cells. This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using HER2/ErbB2,p185-Specific Recombinant antibody (84046-1-RR)

    IHC staining of human ovarian cancer using 84046-1-RR (same clone as 84046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 84046-1-RR (HER2/ErbB2,p185-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse uterus tissue using HER2/ErbB2,p185-Specific Recombinant antibody (84046-1-RR)

    IHC staining of mouse uterus using 84046-1-RR (same clone as 84046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 84046-1-RR (HER2/ErbB2,p185-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HER2/ErbB2,p185-Specific Recombinant antibody (84046-1-RR)

    IF Staining of MCF-7 using 84046-1-RR (same clone as 84046-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HER2/ErbB2,p185-Specific antibody (84046-1-RR, Clone: 240802C2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HER2/ErbB2,p185-Specific Recombinant monoclonal antibody, PBS Only

HER2/ErbB2,p185-Specific Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84046-1-PBS
Clone No.240802C2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ERBB2, 240802C2, CD340, HER 2, HER 2/neu

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84046-1-PBS targets HER2/ErbB2,p185-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Calculated molecular weight 138 kDa
Observed molecular weight185 kDa
GenBank accession numberBC167147
Gene Symbol HER2/ErbB2
Gene ID (NCBI) 2064
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP04626
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.

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