Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, HT-29 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33149-1-AP targets EPS8L1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38144 Product name: Recombinant human EPS8L1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 247-596 aa of BC015763 Sequence: LTSDAVALLRDNVTPRENELWTSLGDSWTRPGLELSPEEGPPYRPEFFSGWEPPVTDPQSRAWEDPVEKQLQHERRRRQQSAPQVAVNGHRDLEPESEPQLESETAGKWVLCNYDFQARNSSELSVKQRDVLEVLDDSRKWWKVRDPAGQEGYVPYNILTPYPGPRLHHSQSPARSLNSTPPPPPAPAPAPPPALARPRWDRPRWDSCDSLNGLDPSEKEKFSQMLIVNEELQARLAQGRSGPSRAVPGPRAPEPQLSPGSDASEVRAWLQAKGFSSGTVDALGVLTGAQLFSLQKEELRAVSPEEGARVYSQVTVQRSLLEDKEKVSELEAVMEKQKKKVEGEVEMEVI 相同性解析による交差性が予測される生物種 |
| Full Name | EPS8-like 1 |
| Calculated molecular weight | 80 kDa |
| Observed molecular weight | 80-85 kDa |
| GenBank accession number | BC015763 |
| Gene Symbol | EPS8L1 |
| Gene ID (NCBI) | 54869 |
| RRID | AB_3742872 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8TE68 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EPS8L1 encodes Epidermal Growth Factor Receptor Kinase Substrate 8-like Protein 1, a protein related to EPS8 and positioned within the EPS8 signaling adaptor family. Its biology is centered on signaling at the cell cortex, where it is connected to ruffle membrane and cytosolic compartments and is associated with positive regulation of ruffle assembly. The protein contains multiple interaction-related domains, including a PTB/PI domain, phosphotyrosine-binding domain, SH3 domain, and SAM domain, supporting a role in signal-dependent molecular interactions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EPS8L1 antibody 33149-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |