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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPS8 Recombinant antibody (85184-5-RR)

    WB analysis using 85184-5-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85184-5-RR (EPS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85184-5-RR

    Affinity and Kinetic Characterization of 85184-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85184-5-RR against Human EPS8 were performed. The affinity constant is 27.9 nM.

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EPS8 Recombinant antibody

EPS8 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85184-5-RR
Clone No.242835G12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Epidermal growth factor receptor kinase substrate 8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, C2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85184-5-RR targets EPS8 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EPS8 fusion protein Ag3121 相同性解析による交差性が予測される生物種
Full Name epidermal growth factor receptor pathway substrate 8
Calculated molecular weight 822 aa, 92 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC030010
Gene Symbol EPS8
Gene ID (NCBI) 2059
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12929
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Epidermal growth factor receptor Pathway Substrate 8 (EPS8) is a crucial regulator of the actin cytoskeleton dynamics accompanying cell motility and invasion. This protein contains one PH domain and one SH3 domain leading to its binding activity with multiple cellular targets. EPS8 can function as a unique actin capping protein specifically required for dendritic cell migration and plays roles in the regulation of axonal filopodia in neuronal development and synapse formation. The EPS8 gene may contribute to the development of a subset of colorectal cancers and could have applications in diagnosis and treatment.

Protocols

Product Specific Protocols
WB protocol for EPS8 antibody 85184-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols