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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPRS Monoclonal antibody (67712-1-Ig)

    WB analysis using 67712-1-Ig (same clone as 67712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using EPRS Monoclonal antibody (67712-1-Ig)

    WB analysis of HSC-T6 using 67712-1-Ig (same clone as 67712-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using EPRS Monoclonal antibody (67712-1-Ig)

    WB analysis of PC-12 using 67712-1-Ig (same clone as 67712-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using EPRS Monoclonal antibody (67712-1-Ig)

    WB analysis of NIH/3T3 using 67712-1-Ig (same clone as 67712-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using EPRS Monoclonal antibody (67712-1-Ig)

    IP experiment of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    IP result of anti-EPRS (IP:67712-1-Ig, 4ug; Detection:67712-1-Ig 1:10000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EPRS Monoclonal antibody (67712-1-Ig)

    IHC staining of human lung cancer using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67712-1-Ig (EPRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EPRS Monoclonal antibody (67712-1-Ig)

    IHC staining of human lung cancer using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67712-1-Ig (EPRS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPRS Monoclonal antibody (67712-1-Ig)

    IF Staining of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPRS Monoclonal antibody (67712-1-Ig)

    IF Staining of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BV-2 cells using EPRS Monoclonal antibody (67712-1-Ig)

    IF Staining of BV-2 using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed BV-2 cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

EPRS Monoclonal antibody, PBS Only

EPRS Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 67712-1-PBS
Clone No.1B7G2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EPRS1, EARS, Cell proliferation-inducing gene 32 protein, Bifunctional glutamate/proline--tRNA ligase, Bifunctional aminoacyl-tRNA synthetase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67712-1-PBS targets EPRS in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19184

Product name: Recombinant human EPRS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1163-1512 aa of BC126275

Sequence: RTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY

相同性解析による交差性が予測される生物種
Full Name glutamyl-prolyl-tRNA synthetase
Calculated molecular weight 1512 aa, 171 kDa
Observed molecular weight171 kDa
GenBank accession numberBC126275
Gene Symbol EPRS
Gene ID (NCBI) 2058
RRIDAB_2882902
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP07814
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)