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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPPK1 Polyclonal antibody (31475-1-AP)

    WB analysis using 31475-1-AP (same clone as 31475-1-PBS)

    L02 cells were subjected to Tris-acetate gel system followed by western blot with 31475-1-AP (EPPK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31475-1-PBS in a different storage buffer formulation.

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EPPK1 Polyclonal antibody, PBS Only

EPPK1 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 31475-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EPPK, Epiplakin, EPIPL1, EPIPL

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31475-1-PBS targets EPPK1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPPK1 fusion protein Ag35749 相同性解析による交差性が予測される生物種
Full Name epiplakin 1
Calculated molecular weight555 kDa
Observed molecular weight500-600 kDa
GenBank accession numberNM_031308.3
Gene Symbol EPPK1
Gene ID (NCBI) 83481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP58107
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Epplakin 1 (EPPK1), belonging to the plakin family, is involved in connecting intermediate filaments and regulating their reorganization in stress response. Studies have shown that EPPK1 is involved in the progression of various cancers, such as liver cancer, cervical cancer, and bladder urothelial carcinoma.