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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPOR Polyclonal antibody (32537-1-AP)

    WB analysis using 32537-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32537-1-AP (EPOR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

EPOR Polyclonal antibody

EPOR Polyclonal Antibody for WB, ELISA
Cat No. 32537-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Erythropoietin receptor, EPO-R

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman placenta tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32537-1-AP targets EPOR in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name erythropoietin receptor
Calculated molecular weight55 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_010149.3
Gene Symbol Epor
Gene ID (NCBI) 13857
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDP14753-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Erythropoietin (EPO) receptor (EPOR) is a glycoprotein that belongs to the type I superfamily of single-transmembrane cytokine receptors. It consists of an extracellular domain that binds to the EPO ligand, a transmembrane domain and an intracellular domain. The interaction of EPO and EPOR triggers the activation of several signaling pathways that induce erythropoiesis, including JAK2/STAT5, PI3K/AKT, and MAPK (PMID: 34281163). EPOR is present on erythroid progenitor cells and has also been detected on a wide variety of non-hematopoietic cells (PMID: 36233351). The calculated molecular weight of EPOR is 55 kDa. EPOR undergoes posttranslational modification and the apparent molecular weight of 66-78 kDa has been reported (PMID: 8943308; 12088257).

Protocols

Product Specific Protocols
WB protocol for EPOR antibody 32537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols