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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPN2 Polyclonal antibody (31334-1-AP)

    WB analysis using 31334-1-AP (same clone as 31334-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31334-1-AP (EPN2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31334-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using EPN2 Polyclonal antibody (31334-1-AP)

    IHC staining of mouse lung using 31334-1-AP (same clone as 31334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31334-1-AP (EPN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31334-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using EPN2 Polyclonal antibody (31334-1-AP)

    IHC staining of mouse lung using 31334-1-AP (same clone as 31334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31334-1-AP (EPN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31334-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EPN2 Polyclonal antibody (31334-1-AP)

    IF Staining of HepG2 using 31334-1-AP (same clone as 31334-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPN2 antibody (31334-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31334-1-PBS in a different storage buffer formulation.

EPN2 Polyclonal antibody, PBS Only

EPN2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31334-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EHB21, EPS 15 interacting protein 2, EPS-15-interacting protein 2, epsin 2, Epsin-2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31334-1-PBS targets EPN2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35194

Product name: Recombinant human EPN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 252-448 aa of BC093972

Sequence: LEESRRDTVKIPKKKEHGSLPQQTTLLDLMDALPSSGPAAQKAEPWGPSASTNQTNPWGGPAAPASTSDPWPSFGTKPAASIDPWGVPTGATAQSVPKNSDPWAASQQPASSAGKRASDAWGAVSTTKPVSVSGSFELFSNLNGTIKDDFSEFDNLRTSKKTAESVTSLPSQNNGTTSPDPFESQPLTVASSKPSSA

相同性解析による交差性が予測される生物種
Full Name epsin 2
Calculated molecular weight 584 aa, 62 kDa
Observed molecular weight68 kDa
GenBank accession numberBC093972
Gene Symbol EPN2
Gene ID (NCBI) 22905
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDO95208
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EPN2 (EPS 15 interacting protein 2) is a protein that interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. EPN2 is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery.

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