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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPN1 Polyclonal antibody (14469-1-AP)

    WB analysis using 14469-1-AP (same clone as 14469-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14469-1-AP (EPN1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14469-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPN1 Polyclonal antibody (14469-1-AP)

    IF Staining of HeLa using 14469-1-AP (same clone as 14469-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPN1 antibody (14469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14469-1-PBS in a different storage buffer formulation.

EPN1 Polyclonal antibody, PBS Only

EPN1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 14469-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14469-1-PBS targets EPN1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5838

Product name: Recombinant human EPN1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 204-550 aa of BC044651

Sequence: RIRRGDDLRLQMAIEESKRETGGKEESSLMDLADVFTAPAPAPTTDPWGGPAPMAAAVPTAAPTSDPWGGPPVPPAADPWGGPAPTPASGDPWRPAAPAGPSVDPWGGTPAPAAGEGPTPDPWGSSDGGVPVSGPSASDPWTPAPAFSDPWGGSPAKPSTNGTTAGGFDTEPDEFSDFDRLRTALPTSGSSAGELELLAGEVPARSPGAFDMSGVRGSLAEAVGSPPPAATPTPTPPTRKTPESFLGPNAALVDLDSLVSRPGPTPPGAKASNPFLPGGGPATGPSVTNPFQPAPPATLTLNQLRLSPVPPVPGAPPTYISPLGGGPGLPPMMPPGPPAPNTNPFLL

相同性解析による交差性が予測される生物種
Full Name epsin 1
Calculated molecular weight 60 kDa
Observed molecular weight65 kDa
GenBank accession numberBC044651
Gene Symbol EPN1
Gene ID (NCBI) 29924
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6I3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Epsin 1 (EPN1) belongs to the epsin family, which also includes EPN2 and EPN3. They belong to the endocytic clathrin family and are involved in receptor endocytosis. EPN1 is widely and highly expressed in tumors.

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