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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of HEK-293 using 66071-1-Ig (same clone as 66071-1-PBS)

    WB result of EPLIN antibody (66071-1-Ig, 1:2000) with si-Control and si-EPLIN transfected HEK293 cells. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of HeLa using 66071-1-Ig (same clone as 66071-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of human brain using 66071-1-Ig (same clone as 66071-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of HEK-293 using 66071-1-Ig (same clone as 66071-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of COLO 320 using 66071-1-Ig (same clone as 66071-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using EPLIN Monoclonal antibody (66071-1-Ig)

    IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using EPLIN Monoclonal antibody (66071-1-Ig)

    IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPLIN Monoclonal antibody (66071-1-Ig)

    IF Staining of HeLa using 66071-1-Ig (same clone as 66071-1-PBS)

    Immunofluorescent analysis of Hela cells using 66071-1-Ig (EPLIN antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EPLIN Monoclonal antibody, PBS Only

EPLIN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66071-1-PBS
Clone No.6E6E6

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

LIMA1, 6E6E6, LIM domain and actin binding 1, LIM domain and actin-binding protein 1, PP624

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66071-1-PBS targets EPLIN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag13288

Product name: Recombinant human LIMA1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 261-401 aa of BC001247

Sequence: KPLSPDSRASSLSESSPPKAMKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAK

相同性解析による交差性が予測される生物種
Full Name LIM domain and actin binding 1
Calculated molecular weight 85 kDa
Observed molecular weight 90 kDa, 110 kDa
GenBank accession numberBC001247
Gene Symbol EPLIN
Gene ID (NCBI) 51474
RRIDAB_11043341
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UHB6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.

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