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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using EPLIN Polyclonal antibody (16639-1-AP)

    WB analysis of HEK-293 using 16639-1-AP (same clone as 16639-1-PBS)

    WB result of EPLIN antibody (16639-1-AP, 1:1000) with si-Control and si-EPLIN transfected HEK293 cells. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using EPLIN Polyclonal antibody (16639-1-AP)

    WB analysis of HUVEC using 16639-1-AP (same clone as 16639-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 16639-1-AP (EPLIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using EPLIN Polyclonal antibody (16639-1-AP)

    WB analysis of human placenta using 16639-1-AP (same clone as 16639-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16639-1-AP (EPLIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using EPLIN Polyclonal antibody (16639-1-AP)

    IP experiment of mouse liver using 16639-1-AP (same clone as 16639-1-PBS)

    IP result of anti-EPLIN (IP:16639-1-AP, 4ug; Detection:16639-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EPLIN Polyclonal antibody (16639-1-AP)

    IHC staining of human prostate cancer using 16639-1-AP (same clone as 16639-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EPLIN Polyclonal antibody (16639-1-AP)

    IHC staining of human pancreas cancer using 16639-1-AP (same clone as 16639-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16639-1-AP (EPLIN antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using EPLIN Polyclonal antibody (16639-1-AP)

    IF Staining of PC-3 using 16639-1-AP (same clone as 16639-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EPLIN Polyclonal antibody (16639-1-AP)

    IF Staining of HepG2 using 16639-1-AP (same clone as 16639-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EPLIN Polyclonal antibody (16639-1-AP)

    IF Staining of HepG2 using 16639-1-AP (same clone as 16639-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using LIMA1 antibody 16639-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

EPLIN Polyclonal antibody, PBS Only

EPLIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16639-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

LIMA1, SREBP3, PP624, LIM domain and actin-binding protein 1, LIM domain and actin binding 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16639-1-PBS targets EPLIN in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPLIN fusion protein Ag9994 相同性解析による交差性が予測される生物種
Full Name LIM domain and actin binding 1
Calculated molecular weight 85 kDa
Observed molecular weight 90 kDa, 110 kDa
GenBank accession numberBC001247
Gene Symbol EPLIN
Gene ID (NCBI) 51474
RRIDAB_2136657
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHB6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. The 110 kDa EPLIN-β isoform represents the full-length protein and the 90 kDa EPLIN-α isoform lacks the amino-terminal 160 amino acids. EPLIN-a expression is often down-regulated in cancerous cells and tissues. (PMID: 10806352, 31644899)