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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using EPHX2 Monoclonal antibody (67322-1-Ig)

    WB analysis of A549 using 67322-1-Ig (same clone as 67322-1-PBS)

    WB result of EPHX2 antibody (67322-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX2 transfected A549 cells. This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EPHX2 Monoclonal antibody (67322-1-Ig)

    WB analysis using 67322-1-Ig (same clone as 67322-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67322-1-Ig (EPHX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EPHX2 Monoclonal antibody (67322-1-Ig)

    IHC staining of human breast cancer using 67322-1-Ig (same clone as 67322-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67322-1-Ig (EPHX2 antibody) at dilution of 1:4000 (under 10x lens). This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EPHX2 Monoclonal antibody (67322-1-Ig)

    IHC staining of human breast cancer using 67322-1-Ig (same clone as 67322-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67322-1-Ig (EPHX2 antibody) at dilution of 1:4000 (under 40x lens). This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using EPHX2 Monoclonal antibody (67322-1-Ig)

    IF Staining of A549 using 67322-1-Ig (same clone as 67322-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using EPHX2 antibody (67322-1-Ig, Clone: 4H6C8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EPHX2 Monoclonal antibody, PBS Only

EPHX2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67322-1-PBS
Clone No.4H6C8

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67322-1-PBS targets EPHX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4870

Product name: Recombinant human EPHX2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 212-555 aa of BC013874

Sequence: ELEKVTGIQLLNTPAPLPTSCNPSDMSHGYVTVKPRVRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEAELEQNLSRTFKSLFRASDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKILIPALMVTAEKDFVLVPQMSQHMEDWIPHLKRGHIEDCGHWTQMDKPTEVNQILIKWLDSDARNPPVVSKM

相同性解析による交差性が予測される生物種
Full Name epoxide hydrolase 2, cytoplasmic
Calculated molecular weight 63 kDa
Observed molecular weight 56-63 kDa
GenBank accession numberBC013874
Gene Symbol EPHX2
Gene ID (NCBI) 2053
RRIDAB_2882582
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP34913
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).

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