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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using EPHB1 Monoclonal antibody (67080-1-Ig)

    WB analysis of mouse cerebellum using 67080-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using EPHB1 Monoclonal antibody (67080-1-Ig)

    WB analysis of fetal human brain using 67080-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using EPHB1 Monoclonal antibody (67080-1-Ig)

    WB analysis of mouse brain using 67080-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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EPHB1 Monoclonal antibody

EPHB1 Monoclonal Antibody for WB, ELISA
Cat No. 67080-1-Ig
Clone No.1B7C12
Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse and More (1)

Applications

WB, ELISA

Synonyms

EK6, ELK, EPH like kinase 6, EPH receptor B1, EPHB1, Ephrin type B receptor 1, EPHT2, FLJ37986, Hek6, NET

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse cerebellum tissue, mouse brain tissue, fetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67080-1-Ig targets EPHB1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivity rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EPHB1 fusion protein Ag16347 相同性解析による交差性が予測される生物種
Full Name EPH receptor B1
Calculated molecular weight 984 aa, 110 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC111744
Gene Symbol EPHB1
Gene ID (NCBI) 2047
RRIDAB_2882388
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54762
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

EPHB1, also named as EPHT2, NET, HEK6 and ELK, belongs to the protein kinase superfamily, Tyr protein kinase family, and Ephrin receptor subfamily. It is a receptor for members of the ephrin-B family. EPHB1 binds to ephrin-B1, -B2 and -B3. EPHB1 may be involved in cell-cell interactions in the nervous system. EPHB1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Protocols

Product Specific Protocols
WB protocol for EPHB1 antibody 67080-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Vis

EphB1 causes retinal damage through inflammatory pathways in the retina and retinal Müller cells

Authors - Li Liu
View Article