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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using EPHA7 Monoclonal antibody (66667-1-Ig)

    WB analysis of human brain using 66667-1-Ig (same clone as 66667-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66667-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using EPHA7 Monoclonal antibody (66667-1-Ig)

    WB analysis of pig brain using 66667-1-Ig (same clone as 66667-1-PBS)

    Untreated and PNGase F-treated lysates of pig brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66667-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using EPHA7 Monoclonal antibody (66667-1-Ig)

    WB analysis of rat brain using 66667-1-Ig (same clone as 66667-1-PBS)

    Untreated and PNGase F-treated lysates of rat brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66667-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using EPHA7 Monoclonal antibody (66667-1-Ig)

    WB analysis of pig brain using 66667-1-Ig (same clone as 66667-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66667-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using EPHA7 Monoclonal antibody (66667-1-Ig)

    WB analysis of rat brain using 66667-1-Ig (same clone as 66667-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66667-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using EPHA7 Monoclonal antibody (66667-1-Ig)

    WB analysis of mouse cerebellum using 66667-1-Ig (same clone as 66667-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66667-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EPHA7 Monoclonal antibody, PBS Only

EPHA7 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66667-1-PBS
Clone No.1A7E5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, Indirect ELISA

Synonyms

EPH homology kinase 3, EK11, EHK-3, EHK3, EHK 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66667-1-PBS targets EPHA7 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag3813

Product name: Recombinant human EPHA7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 29-279 aa of BC027940

Sequence: AAKEVLLLDSKAQQTELEWISSPPNGWEEISGLDENYTPIRTYQVCQVMEPNQNNWLRTNWISKGNAQRIFVELKFTLRDCNSLPGVLGTCKETFNLYYYETDYDTGRNVRENLYVKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSKKGFYLAFQDVGACIALVSVKVYYKKCWSIIENLAIFPDTVTGSEFSSLVEVRGTCVSSAEEEAENAPRMHCSAEGEWLVPIGKCICKAGYQQKGDTCECK

相同性解析による交差性が予測される生物種
Full Name EPH receptor A7
Calculated molecular weight 998 aa, 112 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC027940
Gene Symbol EPHA7
Gene ID (NCBI) 2045
RRIDAB_2882021
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ15375
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ephrin receptor A7 (EphA7) is a member of the Eph receptor family. EPHA7 plays a role in corticogenesis processes, determines brain size and shape, and is involved in development of the central nervous system (PMID:34176129). In addition, EphA7 also has a dual role of tumor promoter and tumor suppressor. It can participate in cell proliferation, migration and apoptosis through various mechanisms, and afect tumor diferentiation, staging and prognosis. EphA7 may be a potential diagnostic marker and tumor treatment target (PMID:35112312).

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