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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using EPHA5 Polyclonal antibody (55215-1-AP)

    IHC staining of mouse brain using 55215-1-AP (same clone as 55215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55215-1-AP (EPHA5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EPHA5 Polyclonal antibody (55215-1-AP)

    IHC staining of mouse brain using 55215-1-AP (same clone as 55215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55215-1-AP (EPHA5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55215-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using EPHA5 Polyclonal antibody (55215-1-AP)

    IF Staining of SH-SY5Y using 55215-1-AP (same clone as 55215-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 55215-1-AP (EPHA5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55215-1-PBS in a different storage buffer formulation.

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EPHA5 Polyclonal antibody, PBS Only

EPHA5 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 55215-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55215-1-PBS targets EPHA5 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name EPH receptor A5
Calculated molecular weight 115 kDa
GenBank accession numberNM_004439
Gene Symbol EPHA5
Gene ID (NCBI) 2044
RRIDAB_2881289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54756
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.