Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66736 targets EPHA2 in applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | EPHA2 fusion protein Ag22566 相同性解析による交差性が予測される生物種 |
| Full Name | EPH receptor A2 |
| Calculated molecular weight | 976 aa, 108 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC037166 |
| Gene Symbol | EPHA2 |
| Gene ID (NCBI) | 1969 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P29317 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ephrin type-A receptor 2 (EPHA2), belongs to the receptor tyrosine kinases (RTKs) family, that binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (PMID: 11114742). The "reverse signaling" function, whereby the cytoplasmic domain becomes tyrosine phosphorylated, allows interactions with other proteins that may activate signaling pathways in the ligand-expressing cells.