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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPG5 Polyclonal antibody (27666-1-AP)

    WB analysis using 27666-1-AP (same clone as 27666-1-PBS)

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 27666-1-AP (EPG5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EPG5 Polyclonal antibody (27666-1-AP)

    IHC staining of human kidney using 27666-1-AP (same clone as 27666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27666-1-AP (KIAA1632 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 27666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EPG5 Polyclonal antibody (27666-1-AP)

    IHC staining of human kidney using 27666-1-AP (same clone as 27666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27666-1-AP (KIAA1632 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 27666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EPG5 Polyclonal antibody (27666-1-AP)

    IHC staining of human lung cancer using 27666-1-AP (same clone as 27666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27666-1-AP (EPG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27666-1-PBS in a different storage buffer formulation.

EPG5 Polyclonal antibody, PBS Only

EPG5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27666-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

HEEW1, KIAA1632, UPF0493 protein KIAA1632

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27666-1-PBS targets EPG5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24801

Product name: Recombinant human KIAA1632 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2112-2462 aa of BC130614

Sequence: ETRSMIVCLLFMMILLAKEVQLVDQTDSPLLSLLGQTSSLSWHLVDIVSYQSVLSYFSSHYPPSIILAKESYAELIMKLLKVSAGLSIPTDSQKHLDAVPKCQAFTHQMVQFLSTLEQNGKITLAVLEQEMSKLLDDIIVFNPPDMDSQTRHMALSSLFMEVLMMMNNATIPTAEFLRGSIRTWIGQKMHGLVVLPLLTAACQSLASVRHMAETTEACITAYFKESPLNQNSGWGPILVSLQVPELTMEEFLQECLTLGSYLTLYVYLLQCLNSEQTLRNEMKVLLILSKWLEQVYPSSVEEEAKLFLWWHQVLQLSLIQTEQNDSVLTESVIRILLLVQSRQNLVAEERL

相同性解析による交差性が予測される生物種
Full Name KIAA1632
Calculated molecular weight 2579 aa, 292 kDa
Observed molecular weight270-290 kDa
GenBank accession numberBC130614
Gene Symbol EPG5
Gene ID (NCBI) 57724
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCE0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EPG5, a metazoan-specific autophagy protein, regulates autophagy by promoting fusion of autophagosomes with lysosomes and/or late endosomes. EPG5 regulates the stability and the assembly of STX17-SNAP29-VAMP8 trans-SNARE complexes. Loss of function of EPG5 causes non-specific fusion of autophagosomes with other endocytic vesicles, resulting in formation of non-degradative enlarged vesicles. EPG5 expression in human pluripotent stem cells like ESC and iPSC is higher than that of human somatic cells.(PMID: 30931944, PMID: 27588602)

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