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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPCR/CD201 Recombinant antibody (84494-2-RR)

    WB analysis using 84494-2-RR (same clone as 84494-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84494-2-RR (EPCR/CD201 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84494-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01323-3

    Sandwich ELISA standard curve of MP01323-3

    Sandwich ELISA standard curve of MP01323-3, Mouse PROCR Recombinant Matched Antibody Pair - PBS only. 84494-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1517. 84494-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP01323-3

    Sample test of MP01323-3

    The mean PROCR concentration was determined to be 469,591.3 pg/mL in bEnd.3 cell extract based on a 1.8 mg/mL extract load and 30,645.7 pg/mL in mouse lung tissue extract based on a 1.6 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of mouse placenta tissue using EPCR/CD201 Recombinant antibody (84494-2-RR)

    IF Staining of mouse placenta using 84494-2-RR (same clone as 84494-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse placenta tissue using EPCR/CD201 antibody (84494-2-RR, Clone: 241853A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84494-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01323-3

    Cytometric bead array standard curve of MP01323-3

    Cytometric bead array standard curve of MP01323-3, MOUSE EPCR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84494-2-PBS. Detection antibody: 84494-3-PBS. Standard: Eg1517. Range: 0.469-60 ng/mL.

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Mouse EPCR/CD201 Recombinant antibody, PBS Only (Capture)

EPCR/CD201 Uni-rAbTM Recombinant Antibody for WB, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84494-2-PBS
Clone No.241853A9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241853A9, Activated protein C receptor, APC receptor, CD201, Centrocyclin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84494-2-PBS targets EPCR/CD201 as part of a matched antibody pair:

MP01323-2: 84494-2-PBS capture and 84494-4-PBS detection (validated in Cytometric bead array)

MP01323-3: 84494-2-PBS capture and 84494-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EPCR/CD201 fusion protein Eg1517 相同性解析による交差性が予測される生物種
Full Name protein C receptor, endothelial
Calculated molecular weight27kDa
Observed molecular weight40-50 kDa
GenBank accession numberNM_011171.2
Gene Symbol PROCR
Gene ID (NCBI) 19124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ64695
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Protein C receptor (PROCR), also known as activated protein C receptor (APC receptor), EPCR, or CD201, is a transmembrane glycoprotein. EPCR promotes the activation of protein C, which has anticoagulant and cytoprotective effects. EPCR facilitates the activation of protein C by the thrombin-thrombomodulin complex but also facilitates APC-mediated cytoprotective impact on cells that involve the activation of protease-activated receptors (PAR) 1 and 3(PMID: 27207424).