Validation Data Gallery
Tested Applications
| Positive WB detected in | human red blood cells, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
16808-1-AP targets Dematin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10300 Product name: Recombinant human EPB49 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-405 aa of BC017445 Sequence: MERLQKQPLTSPGSVSPSRDSSVPGSPSSIVAKMDNQVLGYKDLAAIPKDKAILDIERPDLMIYEPHFTYSLLEHVELPRSRERSLSPKSTSPPPSPEVWADSRSPGIISQASAPRTTGTPRTSLPHFHHPETSRPDSNIYKKPPIYKQRESVGGSPQTKHLIEDLIIESSKFPAAQPPDPNQPAKIETDYWPCPPSLAVVETEWRKRKASRRGAEEEEEEEDDDSGEEMKALRERQREELSKVTSNLGKMILKEEMEKSLPIRRKTRSLPDRTPFHTSLHQGTSKSSSLPAYGRTTLSRLQSTEFSPSGSETGSPGLQNGEGQRGRMDRGNSLPCVLEQKIYPYEMLVVTNKGRTKLPPGVDRMRLERHLSAEDFSRVFAMSPEEFGKLALWKRNELKKKASLF 相同性解析による交差性が予測される生物種 |
| Full Name | erythrocyte membrane protein band 4.9 (dematin) |
| Calculated molecular weight | 405 aa, 46 kDa |
| Observed molecular weight | 48-52 kDa |
| GenBank accession number | BC017445 |
| Gene Symbol | Dematin |
| Gene ID (NCBI) | 2039 |
| RRID | AB_2277891 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08495 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Dematin (band 4.9) is a widely expressed cytoskeletal protein which regulates cell shape and cell migration. It is best known for its role within the red blood cell (RBC), where it has been localized to the spectrin-actin junctions of RBC membrane. Dematin has a 48 kDa subunit containing a headpiece domain and a 52 kDa subunit.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Dematin antibody 16808-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Med Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome | ||
Exp Cell Res Dematin inhibits glioblastoma malignancy through RhoA-mediated CDKs downregulation and cytoskeleton remodeling. |
