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Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using EPB41L3 Recombinant antibody (83275-2-RR)

    WB analysis of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 83275-2-RR (EPB41L3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83275-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using EPB41L3 Recombinant antibody (83275-2-RR)

    WB analysis of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

    WB result of EPB41L3 antibody (83275-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPB41L3 transfected U2OS cells. This data was developed using the same antibody clone with 83275-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using EPB41L3 Recombinant antibody (83275-2-RR)

    FC experiment of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug EPB41L3 Recombinant antibody (83275-2-RR, Clone:230501D7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83275-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83275-2-RR

    Affinity and Kinetic Characterization of 83275-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83275-2-RR against Human EPB41L3 were performed. The affinity constant is below 1 pM.

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  • KD/KO Validated

EPB41L3 Recombinant antibody, PBS Only

EPB41L3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83275-2-PBS
Clone No.230501D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3, 4.1B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83275-2-PBS targets EPB41L3 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EPB41L3 fusion protein Ag1089 相同性解析による交差性が予測される生物種
Full Name erythrocyte membrane protein band 4.1-like 3
Calculated molecular weight 121 kDa
Observed molecular weight120-130 kDa
GenBank accession numberBC008377
Gene Symbol EPB41L3
Gene ID (NCBI) 23136
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y2J2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EPB41L3, also known as 4.1B and DAL1, belongs to the protein 4.1 family. It is a tumor suppressor that inhibits cell proliferation and promotes apoptosis. EPB41L3 modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5 (PMID: 15334060, 15737618). DAL1 loss is an early event in meningioma tumorigenesis (PMID: 10888600).