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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using EPB41L2 Polyclonal antibody (15437-1-AP)

    WB analysis of HeLa using 15437-1-AP (same clone as 15437-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15437-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using EPB41L2 Polyclonal antibody (15437-1-AP)

    WB analysis of Jurkat using 15437-1-AP (same clone as 15437-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15437-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using EPB41L2 Polyclonal antibody (15437-1-AP)

    IP experiment of HEK-293 using 15437-1-AP (same clone as 15437-1-PBS)

    IP result of anti-EPB41L2 (IP:15437-1-AP, 4ug; Detection:15437-1-AP 1:4000) with HEK-293 cells lysate 1610 ug. This data was developed using the same antibody clone with 15437-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EPB41L2 Polyclonal antibody (15437-1-AP)

    IHC staining of human ovary tumor using 15437-1-AP (same clone as 15437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15437-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EPB41L2 Polyclonal antibody (15437-1-AP)

    IHC staining of human ovary tumor using 15437-1-AP (same clone as 15437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15437-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using EPB41L2 Polyclonal antibody (15437-1-AP)

    IF Staining of A431 using 15437-1-AP (same clone as 15437-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EPB41L2 antibody (15437-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 15437-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPB41L2 Polyclonal antibody (15437-1-AP)

    IF Staining of HeLa using 15437-1-AP (same clone as 15437-1-PBS)

    Immunofluorescent analysis of HeLa cells using 15437-1-AP (EPB41L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15437-1-PBS in a different storage buffer formulation.

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EPB41L2 Polyclonal antibody, PBS Only

EPB41L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15437-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Erythrocyte membrane protein band 4.1-like 2, EPB41 L2, Band 4.1-like protein 2, Band 4.1 like protein 2, 4.1G

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15437-1-PBS targets EPB41L2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPB41L2 fusion protein Ag3647 相同性解析による交差性が予測される生物種
Full Name erythrocyte membrane protein band 4.1-like 2
Calculated molecular weight 112 kDa
Observed molecular weight 130-150 kDa
GenBank accession numberBC034718
Gene Symbol EPB41L2
Gene ID (NCBI) 2037
RRIDAB_10951110
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43491
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EPB41L2, also known as 4.1G, is a member of the protein 4.1 family, which generally serves as adaptors linking transmembrane proteins to the cytoskeleton(PMID: 21482674).