Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, MCF-7 cells, HeLa cells, Jurkat cells, RAW 264.7 cells, PC-3 cells |
It is recommended to use NETN lysis buffer for sample preparation during WB detection.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 28 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 3 publications below |
| ChIP | See 1 publications below |
Product Information
20695-1-AP targets p300 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | E1A binding protein p300 |
| Calculated molecular weight | 264 kDa |
| Observed molecular weight | 300 kDa |
| GenBank accession number | NM_001429 |
| Gene Symbol | EP300 |
| Gene ID (NCBI) | 2033 |
| RRID | AB_3085614 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q09472 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for p300 antibody 20695-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Exercise-induced histone lactylation in monocyte-derived macrophages restores cardiac immune homeostasis and function in sepsis-induced cardiomyopathy. | ||
Free Radic Biol Med METTL3-mediated m6A modification of TIFA mRNA promotes tubular cell pyroptosis in acute kidney injury. | ||
Mol Med Rep lncRNA NEAT1 promotes the proliferation of hemangioma cells by transcriptionally activating β‑catenin via enhancing H3K18 lactylation.
| ||
Adv Sci (Weinh) Lactylation Enhances the Activity of Lactate Dehydrogenase A and Promotes the Chemoresistance to Cisplatin Through Facilitating DNA Nonhomologous End Junction in Lung Adenocarcinoma. | ||
Cell Metab Targeting gut microbial nitrogen recycling and cellular uptake of ammonium to improve bortezomib resistance in multiple myeloma | ||
J Med Chem Discovery of Novel CBP/p300 and BRD4 Dual-Target PROTACs with Potent Antitumor Activity in Prostate Cancer. |