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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ENTPD8 Polyclonal antibody (21010-1-AP)

    WB analysis using 21010-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using ENTPD8 Polyclonal antibody (21010-1-AP)

    WB analysis of rat liver using 21010-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ENTPD8 Polyclonal antibody (21010-1-AP)

    WB analysis using 21010-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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ENTPD8 Polyclonal antibody

ENTPD8 Polyclonal Antibody for WB, ELISA
Cat No. 21010-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, ELISA

Synonyms

E NTPDase 8, ENTPD8, GLSR2492, NTPDase 8, NTPDase8, UNQ2492

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in37°C incubated HepG2 cells, mouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21010-1-AP targets ENTPD8 in WB, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENTPD8 fusion protein Ag15224 相同性解析による交差性が予測される生物種
Full Name ectonucleoside triphosphate diphosphohydrolase 8
Calculated molecular weight 495 aa, 54 kDa
Observed molecular weight 70 kDa, 54 kDa
GenBank accession numberBC141810
Gene Symbol ENTPD8
Gene ID (NCBI) 377841
RRIDAB_10755288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5MY95
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ENTPD8 (Ectonucleoside triphosphate diphosphohydrolase 8) is also named as E-NTPDase 8, NTPDase8 and belongs to the GDA1/CD39 NTPase family. NTPDase8 is the canalicular ecto-ATPase/ATPDase and is responsible for the main hepatic NTP-Dase activity(PMID:17095758). The estimated molecular mass of expressed human ENTPD8 is 70-85 kDa which is N-glycosylated and after deglycosylation, it has a nearly identical molecular mass, 54 kDa(PMID:16752921;7781224). It has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 49 kDa. This antibody is specific to ENTPD8.

Protocols

Product Specific Protocols
WB protocol for ENTPD8 antibody 21010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols