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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ENTPD7 Polyclonal antibody

ENTPD7 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24558-1-AP

Synonyms

ENTPD7, LALP1, NTPDase 7, RP11 483F11.1

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 24558-1-AP

    WB analysis of HeLa using 24558-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24558-1-AP (ENTPD7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 24558-1-AP

    IHC staining of human ovary tumor using 24558-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24558-1-AP (ENTPD7 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 24558-1-AP

    IHC staining of human ovary tumor using 24558-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24558-1-AP (ENTPD7 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 24558-1-AP

    IF Staining of HeLa using 24558-1-AP

    Immunofluorescent analysis of HeLa cells using 24558-1-AP (ENTPD7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

24558-1-AP targets ENTPD7 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENTPD7 fusion protein Ag18417 相同性解析による交差性が予測される生物種
Full Name ectonucleoside triphosphate diphosphohydrolase 7
Calculated molecular weight 604 aa, 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC122857
Gene symbol ENTPD7
Gene ID (NCBI) 57089
RRIDAB_2879607
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ectonucleoside triphosphate diphosphohydrolase 7 (ENTPD7), also known as LALP1, is a member of the ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) family. It is located in the nucleoplasm and has an effect on oxidative stress, DNA damage and ageing (PMID: 27737960).

Protocols

Product Specific Protocols
WB protocol for ENTPD7 antibody 24558-1-APDownload protocol
IHC protocol for ENTPD7 antibody 24558-1-APDownload protocol
IF protocol for ENTPD7 antibody 24558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols