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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using ENTPD6 Polyclonal antibody (11626-1-AP)

    WB analysis of mouse spleen using 11626-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11626-1-AP (ENTPD6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ENTPD6 Polyclonal antibody (11626-1-AP)

    IHC staining of human prostate cancer using 11626-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11626-1-AP (ENTPD6 antibody) at dilution of 1:100 (under 10x lens).

ENTPD6 Polyclonal antibody

ENTPD6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11626-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

CD39 antigen like 2, CD39L2, ENTPD6, IL 6SAG, IL6ST2, NTPDase 6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11626-1-AP targets ENTPD6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2148

Product name: Recombinant human ENTPD6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 180-483 aa of BC025980

Sequence: ATAGLRLLPGEKAQKLLQKVKEVFKASPFLVGDDCVSIMNGTDEGVSAWITINFLTGSLKTPGGSSVGMLDLGGGSTQIAFLPRVEGTLQASPPGYLTALRMFNRTYKLYSYSYLGLGLMSARLAILGGVEGQPAKDGKELVSPCLSPSFKGEWEHAEVTYRVSGQKAAASLHELCAARVSEVLQNRVHRTEEVKHVDFYAFSYYYDLAAGVGLIDAEKGGSLVVGDFEIAAKYVCRTLETQPQSSPFSCMDLTYVSLLLQEFGFPRSKVLKLTRKIDNVETSWALGAIFHYIDSLNRQKSPAS

相同性解析による交差性が予測される生物種
Full Name ectonucleoside triphosphate diphosphohydrolase 6 (putative function)
Calculated molecular weight 483 aa, 53 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC025980
Gene Symbol ENTPD6
Gene ID (NCBI) 955
RRIDAB_2100196
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75354
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ENTPD6 antibody 11626-1-APDownload protocol
IHC protocol for ENTPD6 antibody 11626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols