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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using ENTPD1 Recombinant antibody (84235-4-RR)

    WB analysis of human placenta using 84235-4-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84235-4-RR (ENTPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ENTPD1 Recombinant antibody (84235-4-RR)

    IHC staining of human tonsillitis using 84235-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84235-4-RR (ENTPD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ENTPD1 Recombinant antibody (84235-4-RR)

    IHC staining of human placenta using 84235-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84235-4-RR (ENTPD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ENTPD1 Recombinant antibody (84235-4-RR)

    IHC staining of human tonsillitis using 84235-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84235-4-RR (ENTPD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ENTPD1 Recombinant antibody (84235-4-RR)

    IHC staining of human placenta using 84235-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84235-4-RR (ENTPD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD39/ENTPD1 Recombinant monoclonal antibody

CD39/ENTPD1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84235-4-RR
Clone No.241292A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ENTPD1, 241292A1, ATP diphosphohydrolase, ATPDase, ATP-DPH

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman tonsillitis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84235-4-RR targets CD39/ENTPD1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Recombinant Protein

相同性解析による交差性が予測される生物種
Full Name ectonucleoside triphosphate diphosphohydrolase 1
Calculated molecular weight 58 kDa
Observed molecular weight75 kDa
GenBank accession numberBC047664
Gene Symbol CD39
Gene ID (NCBI) 953
RRIDAB_3671788
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP49961
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing.

Protocols

Product Specific Protocols
IHC protocol for CD39/ENTPD1 antibody 84235-4-RRDownload protocol
WB protocol for CD39/ENTPD1 antibody 84235-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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