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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ENT1 Polyclonal antibody (29862-1-AP)

    WB analysis using 29862-1-AP (same clone as 29862-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29862-1-AP (ENT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29862-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ENT1 Polyclonal antibody (29862-1-AP)

    WB analysis of HeLa using 29862-1-AP (same clone as 29862-1-PBS)

    WB result of ENT1 antibody (29862-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENT1 transfected HeLa cells. This data was developed using the same antibody clone with 29862-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ENT1 Polyclonal antibody (29862-1-AP)

    WB analysis using 29862-1-AP (same clone as 29862-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29862-1-AP (ENT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29862-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ENT1 Polyclonal antibody (29862-1-AP)

    IHC staining of human colon cancer using 29862-1-AP (same clone as 29862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29862-1-AP (ENT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29862-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ENT1 Polyclonal antibody (29862-1-AP)

    IHC staining of human lymphoma using 29862-1-AP (same clone as 29862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 29862-1-AP (ENT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29862-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ENT1 Polyclonal antibody, PBS Only

ENT1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29862-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ENT-1, Equilibrative NBMPR-sensitive nucleoside transporter, Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, es nucleoside transporter, Nucleoside transporter, es-type

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29862-1-PBS targets ENT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32003

Product name: Recombinant human ENT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-82 aa of NM_001372327.1

Sequence: NFFMTATQYFTNRLDMSQNVSLVTAELSKDAQASAAPAAPLPERNSLSAIFNN

相同性解析による交差性が予測される生物種
Full Name solute carrier family 29 (nucleoside transporters), member 1
Calculated molecular weight50KD
Observed molecular weight70 kDa
GenBank accession numberNM_001372327.1
Gene Symbol ENT1
Gene ID (NCBI) 2030
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99808
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ENT1 (equilibrative nucleoside transporter 1; also known as SLC29A1) is a ubiquitous protein located at the cell membrane and is a major transporter responsible for the cellular uptake and release of endogenous nucleosides such as adenosine, and nucleoside analogs used in chemotherapy. The predicted molecular weight of ENT1 is 50 kDa, while heavier 54-68 kDa band may be observed due to the glycosylation (PMID: 16448802, 11850433, 16111480). A smaller novel splice variant of the mouse ENT1 has also been identified, which generates a protein of 35-40 kDa (PMID: 18413666).

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